POLQ Polyklonaler Antikörper

POLQ Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28590-1-PBS

Synonyme

POLH, EC:2.7.7.49, DNA polymerase theta, DNA polymerase eta

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  WB analysis using 28590-1-AP (same clone as 28590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

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  WB analysis using 28590-1-AP (same clone as 28590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 28590-1-AP (same clone as 28590-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 28590-1-AP (same clone as 28590-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

28590-1-PBS bindet in WB, IF/ICC, Indirect ELISA POLQ und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLQ fusion protein Ag29643
• Vollständiger Name: polymerase (DNA directed), theta
• Berechnetes Molekulargewicht: 290 kDa
• Beobachtetes Molekulargewicht: 250~290 kDa, 197 kDa
• GenBank-Zugangsnummer: NM_199420
• Gene symbol: POLQ
• Gene ID (NCBI): 10721
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA Polymerase Theta (POLQ) is a DNA polymerase involved in error-prone translesion DNA synthesis (TLS) and error-prone repair of DNA double-strand breaks (DSBs). The enzyme has several unique properties, including low fidelity and the ability to insert and extend past abasic sites and thymine glycol lesions. Accumulating evidence showed that POLQ participates in the development and progression of human cancers because of its critical functions in the repair of genomic double-strand breaks (PMID: 34517891, 27264557). The POLQ cDNA encodes a polypeptide of 2592 amino acids with a predicted mass of 290 kDa, but POLQ is readily proteolyzed with smaller bands at approximately 70 kDa and 110 kDa (PMID: 17334634, 14576298).