POLR1A Polyklonaler Antikörper

POLR1A Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20595-1-PBS

Synonyme

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  WB analysis of HeLa using 20595-1-AP (same clone as 20595-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20595-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20595-1-AP (same clone as 20595-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20595-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 20595-1-AP (same clone as 20595-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20595-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 20595-1-AP (same clone as 20595-1-PBS)

  IP result of anti-POLR1A (IP:20595-1-AP, 4ug; Detection:20595-1-AP 1:300) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 20595-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

20595-1-PBS bindet in WB, IP, Indirect ELISA POLR1A und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLR1A fusion protein Ag14543
• Vollständiger Name: polymerase (RNA) I polypeptide A, 194kDa
• Berechnetes Molekulargewicht: 1720 aa, 195 kDa
• Beobachtetes Molekulargewicht: 190-195 kDa
• GenBank-Zugangsnummer: BC017115
• Gene symbol: POLR1A
• Gene ID (NCBI): 25885
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLR1A, DNA-directed RNA polymerase I subunit RPA1, is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. RNA polymerase I (Pol I) located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I ranscription. Together with the second largest subunit, it is the catalytic core component of RNA polymerase I that synthesizes ribosomal RNA precursors.