POLR2A Rekombinanter Antikörper

POLR2A Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR

Konjugation

Unkonjugiert

CloneNo.

240869E9

Kat-Nr. : 84357-1-PBS

Synonyme

240869E9, 3'-5' exoribonuclease, DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit

---

• 

  WB analysis of COS-7 using 84357-1-RR (same clone as 84357-1-PBS)

  COS-7 cells were subjected to SDS PAGE followed by western blot with 84357-1-RR (POLR2A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84357-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse pancreas using 84357-1-RR (same clone as 84357-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84357-1-RR (POLR2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84357-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 84357-1-RR (same clone as 84357-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2A antibody (84357-1-RR, Clone: 240869E9 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84357-1-PBS in a different storage buffer formulation.

• 

  ChIP experiment of HeLa using 84357-1-RR (same clone as 84357-1-PBS)

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of POLR2A (84357-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 84357-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

84357-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA, ChIP-qPCR POLR2A und zeigt Reaktivität mit Affe, human, Maus

• Getestete Reaktivität: Affe, human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Berechnetes Molekulargewicht: 217 kDa
• Beobachtetes Molekulargewicht: 245 kDa
• GenBank-Zugangsnummer: NM_000937
• Gene symbol: POLR2A
• Gene ID (NCBI): 5430
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA-directed RNA polymerase II subunit RPB1(POLR2A), is part of the core element of the basic RNA polymerase II transcription mechanism. During transcription elongation, Pol II moves on the template as the transcript elongates. Elongation is influenced by the phosphorylation status of the C-terminal domain (CTD) of Pol II largest subunit (RPB1), which serves as a platform for the assembly of factors that regulate transcription initiation, elongation, termination, and mRNA processing.