POLR2D Polyklonaler Antikörper

POLR2D Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 16093-1-AP

Synonyme

DNA-directed RNA polymerase II subunit D, DNA-directed RNA polymerase II subunit RPB4, HSRBP4, HSRPB4, RNA polymerase II RPB4

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  WB analysis using 16093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16093-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 16093-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skeletal muscle using 16093-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16093-1-AP (POLR2D antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 16093-1-AP

  IP result of anti-POLR2D (IP:16093-1-AP, 4ug; Detection:16093-1-AP 1:800) with mouse heart tissue lysate 4000ug.

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  IHC staining of mouse testis using 16093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16093-1-AP (POLR2D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 16093-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 16093-1-AP (POLR2D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, MCF-7-Zellen, Rattenlebergewebe, Ratten-Skelettmuskelgewebe
• Erfolgreiche IP: Mausherzgewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

16093-1-AP bindet in WB, IHC, IP, ELISA POLR2D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLR2D fusion protein Ag8701
• Vollständiger Name: polymerase (RNA) II (DNA directed) polypeptide D
• Berechnetes Molekulargewicht: 142 aa, 16 kDa
• Beobachtetes Molekulargewicht: 16-20 kDa
• GenBank-Zugangsnummer: BC017205
• Gene symbol: POLR2D
• Gene ID (NCBI): 5433
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
mouse	WB	Mol Cell; Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity; Authors - Yuanjun Li
human	WB	Elife; A cryptic K48 ubiquitin chain binding site on UCH37 is required for its role in proteasomal degradation.; Authors - Jiale Du

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

chang wei (Verified Customer) (02-18-2020)

It works well for western blot of Hela S3 whole cell lysates at 1:1000 dilution with incubation at 4 degree overnight.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa S3