POLR2F Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

POLR2F Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15334-1-AP

Synonyme

RPABC14.4, POLRF, HRBP14.4, DNA-directed RNA polymerases I, II, and III subunit RPABC2, DNA-directed RNA polymerases I, II, and III 14.4 kDa polypeptide

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  WB analysis using 15334-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15334-1-AP (POLR2F antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15334-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15334-1-AP (POLR2F antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 15334-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15334-1-AP (POLR2F Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 15334-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15334-1-AP (POLR2F Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 15334-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15334-1-AP (POLR2F Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human cervical cancer using 15334-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 15334-1-AP (POLR2F antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human cervical cancer using 15334-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 15334-1-AP (POLR2F antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human cervical cancer using 15334-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 15334-1-AP (POLR2F Antibody) at dilution of 1:200 (under 10x lens).

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  IF Staining of A431 using 15334-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLR2F antibody (15334-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, C6-Zellen, NIH/3T3-Zellen
• Erfolgreiche Detektion in IHC: humanes Zervixkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

15334-1-AP bindet in WB, IHC, IF/ICC, ELISA POLR2F und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLR2F fusion protein Ag7449
• Vollständiger Name: polymerase (RNA) II (DNA directed) polypeptide F
• Berechnetes Molekulargewicht: 14 kDa
• Beobachtetes Molekulargewicht: 14 kDa
• GenBank-Zugangsnummer: BC003582
• Gene symbol: POLR2F
• Gene ID (NCBI): 5435
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity; Authors - Yuanjun Li
mouse	WB	Cell Biosci; Replication and transcription machinery for ranaviruses: components, correlation, and functional architecture.; Authors - Fei Ke ; KD Validated
mouse	WB	Nat Commun; The transcriptional coactivator RUVBL2 regulates Pol II clustering with diverse transcription factors; Authors - Hui Wang
mouse		Genome Biol; Cross-regulome profiling of RNA polymerases highlights the regulatory role of polymerase III on mRNA transcription by maintaining local chromatin architecture; Authors - Yongpeng Jiang