POLR2G Polyklonaler Antikörper

POLR2G Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18497-1-AP

Synonyme

hRPB19, hsRPB7, POLR2G, RNA polymerase II subunit B7, RPB19, RPB7

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Galerie der Validierungsdaten

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  WB analysis using 18497-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18497-1-AP (POLR2G antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 18497-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18497-1-AP (POLR2G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 18497-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 18497-1-AP (POLR2G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 18497-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 18497-1-AP (POLR2G antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, A375-Zellen, HepG2-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

18497-1-AP bindet in WB, ELISA POLR2G und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLR2G fusion protein Ag13338
• Vollständiger Name: polymerase (RNA) II (DNA directed) polypeptide G
• Berechnetes Molekulargewicht: 172 aa, 19 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC112164
• Gene symbol: POLR2G
• Gene ID (NCBI): 5436
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLR2G (DNA- directed RNA polymerase II subunit G), also known as hRPB19, hsRPB7 and RPB7, is the seventh largest subunit of RNA polymerase II. POLR2G functions in transcription initiation, and is also thought to help stabilize transcribing polyermase molecules during elongation.