POLR2I Polyklonaler Antikörper

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• KD/KO Validated

POLR2I Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 17270-1-AP

Synonyme

DNA-directed RNA polymerase II subunit I, DNA-directed RNA polymerase II subunit RPB9, hRPB14.5, RPB14.5, RPB9

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  WB analysis using 17270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17270-1-AP (POLR2I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse stomach using 17270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse stomach using 17270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17270-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 17270-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat liver using 17270-1-AP

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17270-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2I antibody (17270-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IHC: Maus-Magengewebe, Maushirngewebe, Rattenlebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 2 publications below

Produktinformation

17270-1-AP bindet in WB, IHC, IF/ICC, ELISA POLR2I und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLR2I fusion protein Ag11027
• Vollständiger Name: polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• Berechnetes Molekulargewicht: 125 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC017112
• Gene symbol: POLR2I
• Gene ID (NCBI): 5438
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLR2I, also known as RPB9 or hRPB14.5, is a small core subunit of RNA polymerase II involved in DNA repair and many transcription-related processes (PMID: 21450810). It is expressed in many tissues and may play a key role in the pathological process of hypertensive nephropathy (PMID: 34422001).

Publikationen

Species	Application	Title
mouse	WB	Mol Cell; Targeted protein degradation reveals RNA Pol II heterogeneity and functional diversity; Authors - Yuanjun Li
human	WB	EMBO Rep; The RNA polymerase II subunit Rpb9 activates ATG1 transcription and autophagy; Authors - Ting Huang ; KD Validated