POLR2I Polyklonaler Antikörper

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• KD/KO Validated

POLR2I Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17270-1-PBS

Synonyme

DNA-directed RNA polymerase II subunit I, DNA-directed RNA polymerase II subunit RPB9, hRPB14.5, RPB14.5, RPB9

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  WB analysis using 17270-1-AP (same clone as 17270-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17270-1-AP (POLR2I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 17270-1-AP (same clone as 17270-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2I antibody (17270-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17270-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA POLR2I und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLR2I fusion protein Ag11027
• Vollständiger Name: polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• Berechnetes Molekulargewicht: 125 aa, 15 kDa
• Beobachtetes Molekulargewicht: 15 kDa
• GenBank-Zugangsnummer: BC017112
• Gene symbol: POLR2I
• Gene ID (NCBI): 5438
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLR2I, also known as RPB9 or hRPB14.5, is a small core subunit of RNA polymerase II involved in DNA repair and many transcription-related processes (PMID: 21450810). It is expressed in many tissues and may play a key role in the pathological process of hypertensive nephropathy (PMID: 34422001).