POLR3A Polyklonaler Antikörper

POLR3A Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17530-1-PBS

Synonyme

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  WB analysis using 17530-1-AP (same clone as 17530-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17530-1-AP (POLR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17530-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 17530-1-AP (same clone as 17530-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17530-1-AP (POLR3A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17530-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 17530-1-AP (same clone as 17530-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17530-1-AP (POLR3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17530-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

17530-1-PBS bindet in WB, IHC, Indirect ELISA POLR3A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLR3A fusion protein Ag11536
• Vollständiger Name: polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
• Berechnetes Molekulargewicht: 1390 aa, 156 kDa
• Beobachtetes Molekulargewicht: 140-150 kDa
• GenBank-Zugangsnummer: BC041089
• Gene symbol: POLR3A
• Gene ID (NCBI): 11128
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLR3A encodes the largest subunit of human RNA polymerase III (RPC1), which contributes to the catalytic activity of Pol III and is part of the active centre of the polymerase. Pol III is responsible for the transcription of untranslated RNAs (PMID: 25339210). Bi-allelic missense variants in POLR3A have been associated with phenotypes distinct from WRS: hypogonadotropic hypogonadism and hypomyelinating leukodystrophy with or without oligodontia (PMID: 30414627).