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POLR3A Polyklonaler Antikörper

POLR3A Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 28842-1-PBS

Synonyme

RNA polymerase III subunit C1, hRPC155, EC:2.7.7.6, DNA-directed RNA polymerase III subunit RPC1, DNA-directed RNA polymerase III subunit A

Filter:
  • Western Blot (WB) analysis of various lysates using POLR3A Polyclonal antibody (28842-1-AP)

    WB analysis using 28842-1-AP (same clone as 28842-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28842-1-AP (POLR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28842-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using POLR3A Polyclonal antibody (28842-1-AP)

    IF Staining of A431 using 28842-1-AP (same clone as 28842-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLR3A antibody (28842-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28842-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

28842-1-PBS bindet in WB, IF/ICC, Indirect ELISA POLR3A und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen POLR3A fusion protein Ag29036
Vollständiger Name polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
Berechnetes Molekulargewicht 1390 aa, 156 kDa
Beobachtetes Molekulargewicht155 kDa
GenBank-ZugangsnummerBC041089
Gene symbol POLR3A
Gene ID (NCBI) 11128
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLR3A encodes the largest subunit of human RNA polymerase III (RPC1), which contributes to the catalytic activity of Pol III and is part of the active centre of the polymerase. Pol III is responsible for the transcription of untranslated RNAs (PMID: 25339210). Bi-allelic missense variants in POLR3A have been associated with phenotypes distinct from WRS: hypogonadotropic hypogonadism and hypomyelinating leukodystrophy with or without oligodontia (PMID: 30414627).