POLR3D Monoklonaler Antikörper

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• KD/KO Validated

POLR3D Monoklonal Antikörper für Indirect ELISA, WB

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Kaninchen

Anwendung

Indirect ELISA, WB

Konjugation

Unkonjugiert

CloneNo.

2F5G4

Kat-Nr. : 68263-1-PBS

Synonyme

RPC4, DNA-directed RNA polymerase III subunit RPC4, DNA-directed RNA polymerase III subunit D, BN51T, BN51

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  WB analysis using 68263-1-Ig (same clone as 68263-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68263-1-PBS in a different storage buffer formulation.

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  WB analysis of rabbit brain using 68263-1-Ig (same clone as 68263-1-PBS)

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68263-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 68263-1-Ig (same clone as 68263-1-PBS)

  WB result of POLR3D antibody (68263-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POLR3D transfected HEK-293 cells. This data was developed using the same antibody clone with 68263-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 68263-1-Ig (same clone as 68263-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 68263-1-Ig (POLR3D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68263-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68263-1-Ig (same clone as 68263-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug POLR3D Monoclonal antibody (68263-1-Ig, Clone:2F5G4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68263-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68263-1-PBS bindet in Indirect ELISA, WB POLR3D und zeigt Reaktivität mit human, Kaninchen

• Getestete Reaktivität: human, Kaninchen
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: POLR3D fusion protein Ag7646
• Vollständiger Name: polymerase (RNA) III (DNA directed) polypeptide D, 44kDa
• Berechnetes Molekulargewicht: 44 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC004484
• Gene symbol: POLR3D
• Gene ID (NCBI): 661
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Polymerase (RNA) III (DNA directed) polypeptide D(POLR3D) plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses and acts as nuclear and cytosolic DNA sensor involved in innate immune response. POLR3D can sense non-self dsDNA that serves as template for transcription into dsRNA.