POLR3G Polyklonaler Antikörper

POLR3G Polyklonal Antikörper für IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP,ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24701-1-AP

Synonyme

POLR3G, RNA polymerase III subunit C7, RPC32, RPC7

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Galerie der Validierungsdaten

• 

  WB analysis of HeLa using 24701-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24701-1-AP (POLR3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 24701-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 24701-1-AP (POLR3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 24701-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24701-1-AP (POLR3G Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 24701-1-AP

  IP Result of anti-POLR3G (IP:24701-1-AP, 3ug; Detection:24701-1-AP 1:500) with HeLa cells lysate 2800ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen, K-562-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24701-1-AP bindet in WB, IP,ELISA POLR3G und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POLR3G fusion protein Ag19872
• Vollständiger Name: polymerase (RNA) III (DNA directed) polypeptide G (32kD)
• Berechnetes Molekulargewicht: 223 aa, 26 kDa
• Beobachtetes Molekulargewicht: 26-30 kDa
• GenBank-Zugangsnummer: BC141649
• Gene symbol: POLR3G
• Gene ID (NCBI): 10622
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLR3G also named as RNA polymerase III subunit C7 is a 223 amino acid protein, which belongs to eukaryotic RPC7 RNA polymerase subunit family. The molecular weight of POLR3G is 26 kDa. POLR3G localizes in the nucleus and is a component of the RNA polymerase III complex, which may be involved either in the recruitment and stablization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation.