POMC Polyklonaler Antikörper

POMC Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 22102-1-AP

Synonyme

CLIP, Beta-endorphin, Beta LPH Lipotropin gamma, Adrenocorticotropic hormone, ACTH Melanotropin alpha

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  WB analysis using 22102-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22102-1-AP (POMC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of Transfected HEK-293 using 22102-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 22102-1-AP (ACTH antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pituitary using 22102-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 22102-1-AP (POMC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pituitary using 22102-1-AP

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 22102-1-AP (POMC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22102-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POMC antibody (22102-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mouse pituitary gland tissue, Transfizierte HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Hypophysengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

22102-1-AP bindet in WB, IHC, IF/ICC, ELISA POMC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POMC fusion protein Ag17311
• Vollständiger Name: proopiomelanocortin
• Berechnetes Molekulargewicht: 267 aa, 29 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC065832
• Gene symbol: POMC
• Gene ID (NCBI): 5443
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.

Publikationen

Species	Application	Title
zebrafish	WB	Int J Biol Macromol; Unveiling an indole derivative YM818 as a novel tyrosinase inhibitor with anti-melanogenic and anti-melanin transfer effects; Authors - Chunyan Yang