POMC Monoklonaler Antikörper

POMC Monoklonal Antikörper für IHC, IF/ICC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

3C12C7

Kat-Nr. : 66358-1-PBS

Synonyme

3C12C7, ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma

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  IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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  IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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  IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POMC antibody (66358-1-Ig, Clone: 3C12C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66358-1-Ig (same clone as 66358-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human POMC (66358-1-Ig, Clone:3C12C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66358-1-PBS bindet in IHC, IF/ICC, FC (Intra), ELISA POMC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: POMC fusion protein Ag17311
• Vollständiger Name: proopiomelanocortin
• Berechnetes Molekulargewicht: 267 aa, 29 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC065832
• Gene symbol: POMC
• Gene ID (NCBI): 5443
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.