POMP Rekombinanter Antikörper

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• KD/KO Validated

POMP Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240563D7

Kat-Nr. : 83607-6-RR

Synonyme

PNAS 110, hUMP1, HSPC036, HSPC014, C13orf12

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  WB analysis using 83607-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83607-6-RR (POMP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 83607-6-RR

  rat skin tissue were subjected to SDS PAGE followed by western blot with 83607-6-RR (POMP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 83607-6-RR

  WB result of POMP antibody (83607-6-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POMP transfected HepG2 cells.

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  Affinity and Kinetic Characterization of 83607-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83607-6-RR against Human POMP were performed. The affinity constant is 0.424 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, L02-Zellen, Rattenhautgewebe, SW480-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83607-6-RR bindet in WB, ELISA POMP und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: POMP fusion protein Ag6987
• Vollständiger Name: proteasome maturation protein
• Berechnetes Molekulargewicht: 16 kDa
• Beobachtetes Molekulargewicht: 16 kDa
• GenBank-Zugangsnummer: BC003390
• Gene symbol: POMP
• Gene ID (NCBI): 51371
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Proteasome maturation protein(POMP), is an essential factor of mammalian proteasome biogenesis. It facilitates the main steps in 20S core complex formation at the ER to coordinate the assembly process and to provide cells with freshly formed proteasomes at their site of function. A single nucleotide deletion in this gene causes an autosomal recessive skin disorder, keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK) syndrome.