PON1 Rekombinanter Antikörper

PON1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

241091B7

Kat-Nr. : 83982-2-RR

Synonyme

paraoxonase 1, K-45, EC:3.1.1.2, Aromatic esterase 1, A-esterase 1

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  WB analysis using 83982-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83982-2-RR (PON1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83982-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83982-2-RR against Human PON1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: human plasma

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83982-2-RR bindet in WB, ELISA PON1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PON1 fusion protein Ag12934
• Vollständiger Name: paraoxonase 1
• Berechnetes Molekulargewicht: 355 aa, 40 kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: BC074719
• Gene symbol: PON1
• Gene ID (NCBI): 5444
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PON1, also named PON and K-45, belongs to the paraoxonase family. PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is capable of hydrolyzing a broad spectrum of organophosphate substrates and several aromatic carboxylic acid esters. PON1 may mediate enzymatic protection of low-density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. PON1 was identified as a triple band in a range of approximately 35-40 kDa. For glycosylated, the MW of PON1 is migrated 42-45kd, while the 35 kDa band represents the unglycosylated form (PMID: 17906223, 32002976, 21852972). The antibody has cross-reaction to PON2 and PON3.