PON2 Polyklonaler Antikörper

PON2 Polyklonal Antikörper für FC, IF, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IHC, IF, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 14379-1-AP

Synonyme

A esterase 2, Aromatic esterase 2, paraoxonase 2, PON 2, PON2, Serum aryldialkylphosphatase 2

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Galerie der Validierungsdaten

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  WB analysis of human liver using 14379-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14379-1-AP (PON2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 14379-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14379-1-AP (PON2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 14379-1-AP

  IP Result of anti-PON2 (IP:14379-1-AP, 4ug; Detection:14379-1-AP 1:500) with L02 cells lysate 3200ug.

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  IHC staining of human liver cancer using 14379-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14379-1-AP (PON2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 14379-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14379-1-AP (PON2 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human liver cancer using 14379-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14379-1-AP (PON2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver cancer using 14379-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14379-1-AP (PON2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 14379-1-AP

  Immunofluorescent analysis of HepG2 cells, using PON2 antibody 14379-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 14379-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug PON2 antibody (14379-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: humanes Lebergewebe, L02-Zellen
• Erfolgreiche IP: L02-Zellen
• Erfolgreiche Detektion in IHC: humanes Leberkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HepG2-Zellen
• Erfolgreiche Detektion in FC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200
• Durchflusszytometrie (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

14379-1-AP bindet in WB, IP, IHC, IF, FC, ELISA PON2 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PON2 fusion protein Ag5759
• Vollständiger Name: paraoxonase 2
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: BC046160
• Gene symbol: PON2
• Gene ID (NCBI): 5445
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PON2(Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197).

Publikationen

Species	Application	Title
human	IHC	FASEB J; Breaking the chain at the membrane: paraoxonase 2 counteracts lipid peroxidation at the plasma membrane.; Authors - Henning Hagmann
mouse	WB	Am J Pathol; Expression of myocilin mutants sensitizes cells to oxidative stress-induced apoptosis: implication for glaucoma pathogenesis.; Authors - Joe Myung Kuk MK
human,mouse	IHC	Cells; Paraoxonase 2 (PON2) Deficiency Reproduces Lipid Alterations of Diabetic and Inflammatory Glomerular Disease and Affects TRPC6 Signaling; Authors - Henning Hagmann
mouse	WB,IF	Biomed Pharmacother; GYY4137, a H2S donor, ameliorates kidney injuries in diabetic mice by modifying renal ROS-associated enzymes; Authors - Xueqi Chen