PON2 Rekombinanter Antikörper

PON2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

242673H1

Kat-Nr. : 85126-4-RR

Synonyme

242673H1, A-esterase 2, Aromatic esterase 2, EC:3.1.1.81, PON 2

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  WB analysis using 85126-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85126-4-RR (Pon2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85126-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85126-4-RR (Pon2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, L-929-Zellen, Mauslungengewebe, Rattenherzgewebe, Rattenlungengewebe, RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85126-4-RR bindet in WB, ELISA PON2 und zeigt Reaktivität mit Maus, Ratten

• Getestete Reaktivität: Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PON2 fusion protein Eg2741
• Vollständiger Name: paraoxonase 2
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: NM_183308.2
• Gene symbol: Pon2
• Gene ID (NCBI): 330260
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PON2 (Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197).