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POP1 Polyklonaler Antikörper

POP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12029-1-PBS

Synonyme

POP 1

Filter:
  • Western Blot (WB) analysis of various lysates using POP1 Polyclonal antibody (12029-1-AP)

    WB analysis using 12029-1-AP (same clone as 12029-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12029-1-AP (POP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using POP1 Polyclonal antibody (12029-1-AP)

    WB analysis of HeLa using 12029-1-AP (same clone as 12029-1-PBS)

    WB result of POP1 antibody (12029-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POP1 transfected HeLa cells. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HeLa cells using POP1 Polyclonal antibody (12029-1-AP)

    IP experiment of HeLa using 12029-1-AP (same clone as 12029-1-PBS)

    IP result of anti-POP1 (IP:12029-1-AP, 4ug; Detection:12029-1-AP 1:8000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using POP1 Polyclonal antibody (12029-1-AP)

    IHC staining of human ovary cancer using 12029-1-AP (same clone as 12029-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12029-1-AP (POP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using POP1 Polyclonal antibody (12029-1-AP)

    IF Staining of HepG2 using 12029-1-AP (same clone as 12029-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POP1 antibody (12029-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

12029-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA POP1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen POP1 fusion protein Ag2660
Vollständiger Name processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae)
Berechnetes Molekulargewicht 1024 aa, 115 kDa
Beobachtetes Molekulargewicht 115 kDa
GenBank-ZugangsnummerBC011529
Gene symbol POP1
Gene ID (NCBI) 10940
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Processing of Precursor 1 (POP1), a core component of RNase subunits, is an autoantigen in patients with connective tissue disease and is involved in suppressing inflammation.