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PORCN Polyklonaler Antikörper

PORCN Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20613-1-PBS

Synonyme

DHOF, EC:2.3.1.250, FODH, MG61, por

Filter:
  • Western Blot (WB) analysis of various lysates using PORCN Polyclonal antibody (20613-1-AP)

    WB analysis using 20613-1-AP (same clone as 20613-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20613-1-AP (PORCN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20613-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using PORCN Polyclonal antibody (20613-1-AP)

    IHC staining of rat kidney using 20613-1-AP (same clone as 20613-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20613-1-AP (PORCN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20613-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PORCN Polyclonal antibody (20613-1-AP)

    IHC staining of mouse brain using 20613-1-AP (same clone as 20613-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20613-1-AP (PORCN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20613-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

20613-1-PBS bindet in WB, IHC, Indirect ELISA PORCN und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PORCN fusion protein Ag14664
Vollständiger Name porcupine homolog (Drosophila)
Berechnetes Molekulargewicht 461 aa, 52 kDa
Beobachtetes Molekulargewicht43~50 kDa
GenBank-ZugangsnummerBC019080
Gene symbol PORCN
Gene ID (NCBI) 64840
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PORCN is one of several related membrane-bound O-acyltransferase (MBOAT) enzymes encoding a 461 amino acid-sized (52 kDa) protein named porcupine located in the endoplasmic reticulum and known to be involved in the processing, secretion, and signaling of Wnt proteins (PMID: 17546031). Overexpression of PORCN could promote the proliferation and migration abilities of HCC cells both in vitro and in vivo (PMID: 37588740, 23188502).