Periostin Polyklonaler Antikörper

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• KD/KO Validated

Periostin Polyklonal Antikörper für IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(30)

Kat-Nr. : 19899-1-AP

Synonyme

OSF 2, OSF2, OSF-2, Osteoblast specific factor 2, Osteoblast-specific factor 2

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  IHC staining of human skin cancer using 19899-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19899-1-AP (Periostin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 19899-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 19899-1-AP (Periostin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 19899-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19899-1-AP (Periostin antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 19899-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 19899-1-AP (Periostin antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of human breast cancer using 19899-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19899-1-AP (Periostin antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 19899-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 19899-1-AP (Periostin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Kolongewebe, humanes Mammakarzinomgewebe, humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• IHC: See 11 publications below
• IF: See 22 publications below

Produktinformation

19899-1-AP bindet in IHC, IF, ELISA Periostin und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Periostin fusion protein Ag13791
• Vollständiger Name: periostin, osteoblast specific factor
• Berechnetes Molekulargewicht: 93 kDa
• GenBank-Zugangsnummer: BC106710
• Gene symbol: Periostin
• Gene ID (NCBI): 10631
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, including lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance. The isoforms of periostin are between 83 and 93 kDa in mass and differ in their C-terminal sequences, characterized by individual presence or absence of cassette exons 17-21 (UniProtKB/Swiss-Prot,PMID: 21997759).

Publikationen

Species	Application	Title
mouse	IF	Clin Exp Hypertens; Nuclear receptor subfamily 1 group D member 1 suppresses the proliferation, migration of adventitial fibroblasts, and vascular intimal hyperplasia via mammalian target of rapamycin complex 1/β-catenin pathway; Authors - Ke Peng
human	IF	Matrix Biol; Proteome-wide and matrisome-specific atlas of the human ovary computes fertility biomarker candidates and open the way for precision oncofertility.; Authors - Emna Ouni
rat	IF	Antioxidants (Basel); MicroRNA-4732-3p Is Dysregulated in Breast Cancer Patients with Cardiotoxicity, and Its Therapeutic Delivery Protects the Heart from Doxorubicin-Induced Oxidative Stress in Rats; Authors - Rafael Sánchez-Sánchez
mouse,human	IF	J Nanobiotechnology; Graphene quantum dots rescue angiogenic retinopathy via blocking STAT3/Periostin/ERK signaling.; Authors - Na Zhao
Human	IF	Cell Death Dis; Periostin secreted by cancer-associated fibroblasts promotes cancer stemness in head and neck cancer by activating protein tyrosine kinase 7.; Authors - Binbin Yu
human	IF	Stem Cell Res Ther; Adipose-derived stromal/stem cells are verified to be potential seed candidates for bio-root regeneration in three-dimensional culture.; Authors - Yu Yuan