POT1 Rekombinanter Antikörper

POT1 Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240688E5

Kat-Nr. : 83732-2-PBS

Synonyme

POT1-like telomere end-binding protein, hPot1, 240688E5

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  IF Staining of HeLa using 83732-2-RR (same clone as 83732-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POT1 antibody (83732-2-RR, Clone: 240688E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83732-2-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 83732-2-RR (same clone as 83732-2-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using POT1 antibody (83732-2-RR, Clone: 240688E5 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83732-2-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 83732-2-RR (same clone as 83732-2-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug POT1 Recombinant antibody (83732-2-RR, Clone:240688E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83732-2-PBS in a different storage buffer formulation.

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  FC experiment of SH-SY5Y using 83732-2-RR (same clone as 83732-2-PBS)

  1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug POT1 Recombinant antibody (83732-2-RR, Clone:240688E5) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83732-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00711-1

  Cytometric bead array standard curve of MP00711-1, POT1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83732-4-PBS. Detection antibody: 83732-2-PBS. Standard: Ag0875. Range: 0.78-100 ng/mL.

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  Affinity and Kinetic Characterization of 83732-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83732-2-RR against Human POT1 were performed. The affinity constant is 2.57 nM.

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Geprüfte Anwendungen

Produktinformation

83732-2-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA POT1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: POT1 fusion protein Ag0875
• Vollständiger Name: POT1 protection of telomeres 1 homolog (S. pombe)
• Berechnetes Molekulargewicht: 71 kDa
• GenBank-Zugangsnummer: BC002923
• Gene symbol: POT1
• Gene ID (NCBI): 25913
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Protection of telomeres 1(POT1) is a conserved protein that binds the G-rich stand of its own telomeric repeat sequence, with a role in protecting chromosome ends. It's housekeeping gene that is required to ensure the integrity of chromosome ends in all cells. It's a component of the telomerase ribonucleoprotein(RNP) complex that is esential for the replication of chromosome termini. It consists the TRF1 compex that is involved in the regulation of telomere length by cis-inhibition of telomerase. We have discovered that POT1 exists in at least three consistently occurring forms; 90, 70 and 45kDa. The unexpected molecular weights of POT1 seem to be associated with SUMO1 and ubiquitin conjugation; the latter occurring at a double lysine residue at 289-KK-290 (.PMID: 24054699)