POU2AF1 Monoklonaler Antikörper

POU2AF1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

3C5A7

Kat-Nr. : 66659-1-Ig

Synonyme

3C5A7, B-cell-specific coactivator OBF-1, BOB1, BOB-1, OBF1

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  WB analysis of Raji using 66659-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66659-1-Ig (POU2AF1 antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours.

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  IHC staining of Human tonsillitis using 66659-1-Ig

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66659-1-Ig (POU2AF1 antibody) at dilution of 1:1700 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Raji-Zellen, Daudi-Zellen, Ramos-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:850-1:3400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• ChIP: See 1 publications below

Produktinformation

66659-1-Ig bindet in WB, IHC, ChIP, ELISA POU2AF1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: POU2AF1 fusion protein Ag23613
• Vollständiger Name: POU class 2 associating factor 1
• Berechnetes Molekulargewicht: 256 aa, 27 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC032549
• Gene symbol: POU2AF1
• Gene ID (NCBI): 5450
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.

Publikationen

Species	Application	Title
	ChIP	Cell; YTHDF2 promotes ATP synthesis and immune evasion in B cell malignancies; Authors - Zhenhua Chen