POU4F3 Polyklonaler Antikörper

POU4F3 Polyklonal Antikörper für WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

6

Rezensionen (1)

Kat-Nr. : 21509-1-AP

Synonyme

Brain 3C, Brn 3C, BRN3C, DFNA15, POU class 4 homeobox 3, POU4F3

---

Galerie der Validierungsdaten

• 

  WB analysis of Neuro-2a using 21509-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21509-1-AP (POU4F3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21509-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21509-1-AP (POU4F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 21509-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21509-1-AP (POU4F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, HL-60-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Produktinformation

21509-1-AP bindet in WB, IHC, IF, ELISA POU4F3 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: POU4F3 fusion protein Ag16004
• Vollständiger Name: POU class 4 homeobox 3
• Berechnetes Molekulargewicht: 338 aa, 37 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa
• GenBank-Zugangsnummer: BC104923
• Gene symbol: POU4F3
• Gene ID (NCBI): 5459
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou
mouse	IHC	Front Cell Dev Biol; In vivo Regeneration of Ganglion Cells for Vision Restoration in Mammalian Retinas.; Authors - Dongchang Xiao
mouse	WB,IF	Sci Rep; 661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively.; Authors - Zuberwasim Sayyad
human	WB, IF	Neurochem Int; Human primary retinal cells as an in-vitro model for investigating defective signalling caused by OPTN mutants associated with glaucoma.; Authors - Zuberwasim Sayyad
mouse	IF	Hear Res; Sox2 is required in supporting cells for normal levels of vestibular hair cell regeneration in adult mice; Authors - Amanda N Ciani Berlingeri
mouse		Elife; Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammals; Authors - Amrita A Iyer

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

AMRITA (Verified Customer) (02-15-2021)

The antibody requires a prior antigen retrieval step for 15min in 80 deg centigrade

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Inner ear - organ of Corti hair cells stained

POU4F3 Antibody Immunohistochemistry validation (1:200 dilution) in Inner ear - organ of Corti hair cells stained (Cat no:21509-1-AP)