PPA1 Rekombinanter Antikörper

PPA1 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242963D1

Kat-Nr. : 85445-3-PBS

Synonyme

242963D1, EC:3.6.1.1, Inorganic pyrophosphatase, IOPPP, PP

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  WB analysis using 85445-3-RR (same clone as 85445-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85445-3-RR (PPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85445-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 85445-3-RR (same clone as 85445-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85445-3-RR (PPA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85445-3-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 85445-3-RR (same clone as 85445-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85445-3-RR (PPA1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85445-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85445-3-RR

  Biolayer interferometry (BLl) kinetic assays of 85445-3-RR against Human PPA1 were performed. The affinity constant is 4.07 nM.

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Geprüfte Anwendungen

Produktinformation

85445-3-PBS bindet in WB, IHC, Indirect ELISA PPA1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PPA1 fusion protein Ag6969
• Vollständiger Name: pyrophosphatase (inorganic) 1
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC001022
• Gene symbol: PPA1
• Gene ID (NCBI): 5464
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).