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PPA1 Rekombinanter Antikörper

PPA1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242963D1

Kat-Nr. : 85445-3-RR

Synonyme

242963D1, EC:3.6.1.1, Inorganic pyrophosphatase, IOPPP, PP

Filter:
  • Western Blot (WB) analysis of various lysates using PPA1 Recombinant antibody (85445-3-RR)

    WB analysis using 85445-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85445-3-RR (PPA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PPA1 Recombinant antibody (85445-3-RR)

    IHC staining of human ovary cancer using 85445-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85445-3-RR (PPA1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using PPA1 Recombinant antibody (85445-3-RR)

    IHC staining of human ovary cancer using 85445-3-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85445-3-RR (PPA1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85445-3-RR

    Affinity and Kinetic Characterization of 85445-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85445-3-RR against Human PPA1 were performed. The affinity constant is 4.07 nM.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, A431-Zellen, C6-Zellen, HEK-293T-Zellen, HepG2-Zellen, NIH/3T3-Zellen
Erfolgreiche Detektion in IHChuman ovary cancer tissue
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

85445-3-RR bindet in WB, IHC, ELISA PPA1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PPA1 fusion protein Ag6969
Vollständiger Name pyrophosphatase (inorganic) 1
Berechnetes Molekulargewicht 33 kDa
Beobachtetes Molekulargewicht 33 kDa
GenBank-ZugangsnummerBC001022
Gene symbol PPA1
Gene ID (NCBI) 5464
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PPA1 antibody 85445-3-RRProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen