PPA2 Monoklonaler Antikörper

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• KD/KO Validated

PPA2 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C2E3

Kat-Nr. : 68468-1-PBS

Synonyme

1C2E3, EC:3.6.1.1, HSPC124, PPase 2, Pyrophosphatase SID6 306

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  WB analysis of HEK-293 using 68468-1-Ig (same clone as 68468-1-PBS)

  WB result of PPA2 antibody (68468-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPA2 transfected HEK-293 cells. This data was developed using the same antibody clone with 68468-1-PBS in a different storage buffer formulation.

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  WB analysis using 68468-1-Ig (same clone as 68468-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68468-1-Ig (PPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 68468-1-PBS in a different storage buffer formulation.

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  WB analysis of 4T1 using 68468-1-Ig (same clone as 68468-1-PBS)

  4T1 cells were subjected to SDS PAGE followed by western blot with 68468-1-Ig (PPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68468-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68468-1-PBS bindet in WB, Indirect ELISA PPA2 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PPA2 fusion protein Ag10125
• Vollständiger Name: pyrophosphatase (inorganic) 2
• Berechnetes Molekulargewicht: 305aa,35 kDa; 334aa,38 kDa
• Beobachtetes Molekulargewicht: 34 kDa
• GenBank-Zugangsnummer: BC057219
• Gene symbol: PPA2
• Gene ID (NCBI): 27068
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pyrophosphatase 2 (PPA2) is a mitochondrial-located pyrophosphatase (PPase) that hydrolyzes inorganic pyrophosphate (PPi), generated by numerous nucleotide-dependent reactions, into two orthophosphate molecules (PMID: 34400813). PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function (PMID: 27523597).