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PPAN Polyklonaler Antikörper

PPAN Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11006-1-PBS

Synonyme

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  • Western Blot (WB) analysis of various lysates using PPAN Polyclonal antibody (11006-1-AP)

    WB analysis using 11006-1-AP (same clone as 11006-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11006-1-AP (PPAN antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11006-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using PPAN Polyclonal antibody (11006-1-AP)

    WB analysis of HEK-293 using 11006-1-AP (same clone as 11006-1-PBS)

    WB result of PPAN antibody (11006-1-AP; 1:12000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPAN transfected HEK-293 cells. This data was developed using the same antibody clone with 11006-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using PPAN Polyclonal antibody (11006-1-AP)

    IHC staining of human colon using 11006-1-AP (same clone as 11006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11006-1-AP (PPAN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11006-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

11006-1-PBS bindet in WB, IHC, Indirect ELISA PPAN und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PPAN fusion protein Ag1419
Vollständiger Name peter pan homolog (Drosophila)
Berechnetes Molekulargewicht 53 kDa
Beobachtetes Molekulargewicht 55-58 kDa
GenBank-ZugangsnummerBC009833
Gene symbol PPAN
Gene ID (NCBI) 56342
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPAN (or SSF, SSF1 or SSF2) gene is widely expressed, and encodes peter pan homolog protein which may be localized in nucleus. Two isoforms are produced through alternative splicing. So far the characterization of this protein is largely unknown, multiple phosphorylation sites has been revealed based on high-throughput phosphorylation analysis. This antibody is raised against the C-terminal of huaman PPAN and recognize bands around 55 kDa.