PPAPDC3 Polyklonaler Antikörper

PPAPDC3 Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 20635-1-AP

Synonyme

C9orf67, KIAA0515, NET39, PPAPDC3

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  WB analysis of mouse skeletal muscle using 20635-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20635-1-AP (PPAPDC3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 20635-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20635-1-AP (PPAPDC3 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 20635-1-AP

  IP result of anti-PPAPDC3 (IP:20635-1-AP, 4ug; Detection:20635-1-AP 1:800) with mouse skeletal muscle tissue lysate 4000ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Skelettmuskelgewebe
• Erfolgreiche IP: Maus-Skelettmuskelgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

20635-1-AP bindet in WB, IP, ELISA PPAPDC3 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPAPDC3 fusion protein Ag14676
• Vollständiger Name: phosphatidic acid phosphatase type 2 domain containing 3
• Berechnetes Molekulargewicht: 271 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: BC006362
• Gene symbol: PPAPDC3
• Gene ID (NCBI): 84814
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPAPDC3(Phosphatidic acid phosphatase type 2 domain-containing protein 3) is also named as C9orf67 and belongs to the PA-phosphatase related phosphoesterase family. It plays a role as negative regulator of myoblast differentiation, in part through effects on MTOR signaling.

Publikationen

Species	Application	Title
human	WB	Genome Biol; Specific nuclear envelope transmembrane proteins can promote the location of chromosomes to and from the nuclear periphery.; Authors - Zuleger Nikolaj N
human	WB	Cells; Lamin A/C Assembly Defects in LMNA-Congenital Muscular Dystrophy Is Responsible for the Increased Severity of the Disease Compared with Emery-Dreifuss Muscular Dystrophy.; Authors - Anne T Bertrand
human	WB	Front Cell Dev Biol; Nuclear envelope transmembrane proteins involved in genome organization are misregulated in myotonic dystrophy type 1 muscle; Authors - Vanessa Todorow