PPARA Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PPARA Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (5)

Anwendung

WB, IF, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(199)

Kat-Nr. : 15540-1-AP

Synonyme

hPPAR, NR1C1, PPAR, PPAR alpha, PPARA, PPARα

---

• 

  WB analysis using 15540-1-AP

  C2C12 cells were subjected to SDS PAGE followed by western blot with 15540-1-AP (PPARA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of U-937 using 15540-1-AP

  IP result of anti-PPARA (IP:15540-1-AP, 4ug; Detection:15540-1-AP 1:300) with U-937 cells lysate 4000ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: C2C12-Zellen
• Erfolgreiche IP: U-937-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 13 publications below
• WB: See 187 publications below
• IF: See 13 publications below
• CoIP: See 1 publications below
• ChIP: See 2 publications below

Produktinformation

15540-1-AP bindet in WB, IF, IP, CoIP, ChIP, ELISA PPARA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, hamster, Hausschwein, Huhn, Maus, Ratte, Rind, Ziege
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPARA fusion protein Ag7896
• Vollständiger Name: peroxisome proliferator-activated receptor alpha
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC000052
• Gene symbol: PPARA
• Gene ID (NCBI): 5465
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxisome proliferator-activated receptor alpha (PPARA) is a ligand-activated transcription factor that belongs to the PPAR nuclear receptor superfamily. PPARA is essential in the modulation of lipid transport and metabolism, mainly through activating mitochondrial and peroxisomal fatty acid β-oxidation pathways. In addition, PPARA seems to decrease inflammation mainly through direct interaction with NF-κB, causing inhibition of its signaling pathway or reducing the activated levels of NF-κB and subsequent inflammation. Furthermore, PPARA was implicated in the attenuation of oxidative stress in alcoholic liver disease when treated with polyenephosphatidylcholine through downregulation of ROS-generating enzymes such as ethanol-inducible cytochrome P450 2E1 (CYP2E1), acyl-CoA oxidase, and NADPH oxidase. PPARA exists two isoforms and molecular weight of PPARA isoforms are 52 kDa and 22 kDa. The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily. Among these heterodimers, PPAR:RXR is considered an important signalling mediator of both PPAR ligands, such as fatty acids, and 9-cis retinoic acid (9-cis RA), an RXR ligand. (PMID: 15103326 ). PPARA can form Heterodimer with RXRA and molecular weight of Heterodimer is about 110 kDa.

Publikationen

Species	Application	Title
mouse	IF	Nat Commun; Hyodeoxycholic acid ameliorates nonalcoholic fatty liver disease by inhibiting RAN-mediated PPARα nucleus-cytoplasm shuttling; Authors - Jing Zhong
human	WB	Sci Adv; Small-molecule inhibitor targeting orphan nuclear receptor COUP-TFII for prostate cancer treatment.; Authors - Leiming Wang
mouse	WB	Hepatology; The histone methyltransferase Suv39h2 contributes to nonalcoholic steatohepatitis in mice.; Authors - Zhiwen Fan
mouse	WB	Nat Commun; Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.; Authors - Fangjun Yu
human	WB	Nat Commun; N1-methyladenosine methylation in tRNA drives liver tumourigenesis by regulating cholesterol metabolism.; Authors - Yanying Wang
mouse	WB	Nat Commun; Hepatic HuR modulates lipid homeostasis in response to high-fat diet.; Authors - Zhuojun Zhang