PPARA Monoklonaler Antikörper

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• KD/KO Validated

PPARA Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

1G1E10

Kat-Nr. : 66826-1-PBS

Synonyme

PPAR alpha, PPAR, Peroxisome proliferator-activated receptor alpha, NR1C1, hPPAR

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  WB analysis of ROS1728 using 66826-1-Ig (same clone as 66826-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66826-1-Ig (PPARA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66826-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66826-1-Ig (same clone as 66826-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66826-1-Ig (PPARA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66826-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66826-1-PBS bindet in WB, ELISA PPARA und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PPARA fusion protein Ag7896
• Vollständiger Name: peroxisome proliferator-activated receptor alpha
• Berechnetes Molekulargewicht: 52 kDa
• Beobachtetes Molekulargewicht: 53 kDa
• GenBank-Zugangsnummer: BC000052
• Gene symbol: PPARA
• Gene ID (NCBI): 5465
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxisome proliferator-activated receptor alpha (PPARA) is a ligand-activated transcription factor that belongs to the PPAR nuclear receptor superfamily. PPARA is essential in the modulation of lipid transport and metabolism, mainly through activating mitochondrial and peroxisomal fatty acid β-oxidation pathways. In addition, PPARA seems to decrease inflammation mainly through direct interaction with NF-κB, causing inhibition of its signaling pathway or reducing the activated levels of NF-κB and subsequent inflammation. Furthermore, PPARA was implicated in the attenuation of oxidative stress in alcoholic liver disease when treated with polyenephosphatidylcholine through downregulation of ROS-generating enzymes such as ethanol-inducible cytochrome P450 2E1 (CYP2E1), acyl-CoA oxidase, and NADPH oxidase. PPARA exists two isoforms and molecular weight of PPARA isoforms are 52 kDa and 22 kDa. The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily. Among these heterodimers, PPAR:RXR is considered an important signalling mediator of both PPAR ligands, such as fatty acids, and 9-cis retinoic acid (9-cis RA), an RXR ligand. (PMID: 15103326 ). PPARA can form Heterodimer with RXRA and molecular weight of Heterodimer is about 110 kDa.