PPARD Polyklonaler Antikörper

PPARD Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Kat-Nr. : 10156-2-AP

Synonyme

FAAR, NR1C2, NUC1, NUCI, NUCII, Nuclear hormone receptor 1, PPAR beta, PPAR delta, PPARB, PPARD

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Galerie der Validierungsdaten

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  WB analysis of mouse heart using 10156-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 10156-2-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10156-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10156-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10156-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 10156-2-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 10156-2-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 10156-2-AP (PPARD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 10156-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10156-2-AP (PPARD antibody) at dilution of 1:10 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, COLO 320-Zellen, Maus-Skelettmuskelgewebe
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 20 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

10156-2-AP bindet in WB, IHC, IF, ELISA PPARD und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPARD fusion protein Ag0208
• Vollständiger Name: peroxisome proliferator-activated receptor delta
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC002715
• Gene symbol: PPARD
• Gene ID (NCBI): 5467
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.

Publikationen

Species	Application	Title
mouse	WB	Research (Wash D C); Herpetrione, a New Type of PPARα Ligand as a Therapeutic Strategy Against Nonalcoholic Steatohepatitis; Authors - Lang Linghu
human	WB	EMBO J; The chromatin remodeler SRCAP promotes self-renewal of intestinal stem cells.; Authors - Buqing Ye
		Diabetes; Tissue-specific splicing and dietary interaction of a mutant As160 allele determine muscle metabolic fitness in rodents.; Authors - Xinyu Yang
human	IHC	Cancer Lett; PMID: 32120025; Authors - Fangjia Tong
rat	WB	Eur J Med Chem; Design, synthesis, and biological evaluation of a novel dual peroxisome proliferator-activated receptor alpha/delta agonist for the treatment of diabetic kidney disease through anti-inflammatory mechanisms.; Authors - Kai Liu
rat	WB	Neuropharmacology; PPARβ/δ activation protects against corticosterone-induced ER stress in astrocytes by inhibiting the CpG hypermethylation of microRNA-181a.; Authors - Juan Ji