PPARD Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PPARD Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen

6

CloneNo.

1B10E1

Kat-Nr. : 60193-1-Ig

Synonyme

FAAR, NR1C2, NUC1, NUCI, NUCII, Nuclear hormone receptor 1, PPAR beta, PPAR delta, PPARB, PPARD

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Galerie der Validierungsdaten

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  WB analysis using 60193-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 60193-1-Ig

  WB result of PPARD antibody (60193-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPARD transfected A549 cells.

• 

  WB analysis using 60193-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 60193-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SW480 using 60193-1-Ig

  SW480 cells were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig adipose using 60193-1-Ig

  pig adipose tissue were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, Hausschwein-Fettgewebe, Jurkat-Zellen, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below

Produktinformation

60193-1-Ig bindet in WB, ELISA PPARD und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PPARD fusion protein Ag16770
• Vollständiger Name: peroxisome proliferator-activated receptor delta
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC002715
• Gene symbol: PPARD
• Gene ID (NCBI): 5467
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.

Publikationen

Species	Application	Title
mouse	WB	Nat Commun; A proteomics landscape of circadian clock in mouse liver.; Authors - Yunzhi Wang
mouse	WB	Liver Int; PPARβ/δ activation protects against hepatic ischaemia-reperfusion injury; Authors - Baolin Qian ; KD Validated
mouse	WB	Am J Physiol Endocrinol Metab; Zinc finger protein 407 overexpression upregulates PPAR target gene expression and improves glucose homeostasis in mice; Authors - Alyssa Charrier
mouse	WB	Front Aging Neurosci; A tau fragment links depressive-like behaviors and cognitive declines in Alzheimer's disease mouse models through attenuating mitochondrial function; Authors - Yamei Wang
human	WB	Clin Transl Med; Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma; Authors - Hao Zhang
mouse	WB	iScience; WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation; Authors - Kai Song