PPAR Gamma Polyklonaler Antikörper

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• KD/KO Validated

PPAR Gamma Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 22061-1-AP

Synonyme

CIMT1, NR1C3, PPAR gamma, PPARG, PPARG2

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Galerie der Validierungsdaten

• 

  WB analysis using 22061-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22061-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22061-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 22061-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 22061-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 22061-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 22061-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 22061-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22061-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22061-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22061-1-AP (PPAR gamma antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat colon using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22061-1-AP (PPAR gamma antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, 3T3-L1-Zellen, HL-60-Zellen, humanes Herzgewebe, humanes Hirngewebe, Maus-Eierstockgewebe, Mausherzgewebe, Maushirngewebe, Maushodengewebe, Mauslebergewebe, MCF-7-Zellen, Rattenhirngewebe, Rattenlebergewebe
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Kolonkarzinomgewebe, humanes Plazenta-Gewebe, Ratten-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Produktinformation

22061-1-AP bindet in WB, IHC, ELISA PPAR Gamma und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPAR Gamma fusion protein Ag17136
• Vollständiger Name: peroxisome proliferator-activated receptor gamma
• Berechnetes Molekulargewicht: 505 aa, 58 kDa
• Beobachtetes Molekulargewicht: 66-70 kDa
• GenBank-Zugangsnummer: BC006811
• Gene symbol: PPARG
• Gene ID (NCBI): 5468
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, INS resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 INS-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa). (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).

Publikationen

Species	Application	Title
mouse	WB	Autophagy; Mir223 restrains autophagy and promotes CNS inflammation by targeting ATG16L1.; Authors - Yan Li
mouse	WB	Genes Dev; Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism.; Authors - Stephanie Pearson
mouse	WB	Free Radic Biol Med; Hydrogen sulfide alleviates particulate matter-induced emphysema and airway inflammation by suppressing ferroptosis.; Authors - Ying Wang
mouse	WB	Biochem Pharmacol; A novel pyrazole-containing indolizine derivative suppresses NF-κB activation and protects against TNBS-induced colitis via a PPAR-γ-dependent pathway.; Authors - Yong Fu
		Front Pharmacol; The Active Components of Fuzheng Huayu Formula and Their Potential Mechanism of Action in Inhibiting the Hepatic Stellate Cells Viability - A Network Pharmacology and Transcriptomics Approach.; Authors - Xinrui Xing
rat	WB	Front Pharmacol; Alpha-Asaronol Alleviates Dysmyelination by Enhancing Glutamate Transport Through the Activation of PPARγ-GLT-1 Signaling in Hypoxia-Ischemia Neonatal Rats.; Authors - Yuhang Ge

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