PPAR Gamma Monoklonaler Antikörper

PPAR Gamma Monoklonal Antikörper für WB, IHC, FC (Intra), ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF, FC (Intra), ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

CloneNo.

1F4A2

Kat-Nr. : 66936-1-Ig

Synonyme

PPARG, 1F4A2, NR1C3, Peroxisome proliferator-activated receptor gamma, PPAR-gamma



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHepG2-Zellen, A431-Zellen, A549-Zellen, HL-60-Zellen, K-562-Zellen, MCF-7-Zellen, Maus-Fettgewebe, PC-3-Zellen
Erfolgreiche Detektion in IHChumanes Prostatakarzinomgewebe, humanes Kolonkarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in FC (Intra)HeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunhistochemie (IHC)IHC : 1:250-1:1000
Durchflusszytometrie (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66936-1-Ig bindet in WB, IHC, IF, FC (Intra), ChIP, ELISA PPAR Gamma und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PPAR Gamma fusion protein Ag16657
Vollständiger Name peroxisome proliferator-activated receptor gamma
Berechnetes Molekulargewicht 58 kDa
Beobachtetes Molekulargewicht 50 kDa
GenBank-ZugangsnummerBC006811
Gene symbol PPARG
Gene ID (NCBI) 5468
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, INS resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 INS-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa) (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PPAR Gamma antibody 66936-1-IgProtokoll herunterladen
IHC protocol for PPAR Gamma antibody 66936-1-IgProtokoll herunterladenl
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Acta Pharm Sin B

Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1.

Authors - Piao Luo
ratWB

Phytomedicine

Podophyllotoxin via SIRT1/PPAR /NF-κB axis induced cardiac injury in rats based on the toxicological evidence chain (TEC) concept

Authors - Tao Jiang
mouseWB

Front Immunol

miR-10a-3p modulates adiposity and suppresses adipose inflammation through TGF-β1/Smad3 signaling pathway

Authors - Sonia Kiran
mouseWB,ChIP

Biochem Pharmacol

Flavokawain B is an effective natural peroxisome proliferator-activated receptor γ-selective agonist with a strong glucose-lowering effect

Authors - Qixin Wu
humanWB

Stem Cell Res Ther

Intranasal delivery of hMSC-derived supernatant for treatment of ischemic stroke by inhibiting the pro-inflammatory polarization of neutrophils

Authors - Yixiang Jiang
humanWB

Clin Transl Med

Oestrogen suppresses the adipogenesis of fibro/adipogenic progenitors through reactivating the METTL3-ESR1-mediated loop in post-menopausal females

Authors - Hao Zhou