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PGC1a Monoklonaler Antikörper
PGC1a Monoklonal Antikörper für IF, WB, ELISA
Wirt / Isotyp
Maus / IgG1
Getestete Reaktivität
Hausschwein, human, Maus, Ratte und mehr (4)
Anwendung
WB, IP, IHC, IF, ELISA
Konjugation
Unkonjugiert
CloneNo.
1C1B2
Kat-Nr. : 66369-1-Ig
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | Hausschwein-Skelettmuskelgewebe, C2C12-Zellen, humanes Skelettmuskelgewebe, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe, ROS1728-Zellen |
| Erfolgreiche Detektion in IF | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunfluoreszenz (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| KD/KO | See 8 publications below |
| WB | See 274 publications below |
| IHC | See 19 publications below |
| IF | See 32 publications below |
| IP | See 5 publications below |
| ELISA | See 1 publications below |
Produktinformation
66369-1-Ig bindet in WB, IP, IHC, IF, ELISA PGC1a und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten
| Getestete Reaktivität | Hausschwein, human, Maus, Ratte |
| In Publikationen genannte Reaktivität | human, hamster, Hausschwein, Huhn, Maus, Ratte, Rind, Ziege |
| Wirt / Isotyp | Maus / IgG1 |
| Klonalität | Monoklonal |
| Typ | Antikörper |
| Immunogen | Peptid |
| Vollständiger Name | peroxisome proliferator-activated receptor gamma, coactivator 1 alpha |
| Berechnetes Molekulargewicht | 91 kDa |
| Beobachtetes Molekulargewicht | 70 kDa, 100 kDa |
| GenBank-Zugangsnummer | NM_013261 |
| Gene symbol | PPARGC1A |
| Gene ID (NCBI) | 10891 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein-A-Reinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for PGC1a antibody 66369-1-Ig | Protokoll herunterladen |
| IF protocol for PGC1a antibody 66369-1-Ig | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Sci Adv Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice. | ||
J Hazard Mater Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers | ||
Nat Commun Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance. | ||
Nat Commun METTL3 is essential for postnatal development of brown adipose tissue and energy expenditure in mice. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Michaela (Verified Customer) (11-24-2023) | Worked well in 15μg mouse brain tissue lysate in Western Blot. Band appears between 60-100 kDa
|
FH No (Verified Customer) (10-24-2023) | A good antibody to detect endogenous PGC1a.
 |
FH Mi (Verified Customer) (03-07-2023) | It doesn´t work well in human white and brown adipocyte cells, we got bands at unexpected size. Further KO validation may be needed.
|
FH Huai-Chin (Verified Customer) (07-11-2022) | There are several non-specific bands. At least there's a dominant band after adipogenesis and we can use this antibody at a fairly high dilution.
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FH Mohammad (Verified Customer) (01-21-2020) | It was working with some non-specific bands in lower weight. But after 9 months antibody was degraded in -20.
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