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PGC1a Monoklonaler Antikörper

PGC1a Monoklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte und mehr (5)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(528)

CloneNo.

1C1B2

Kat-Nr. : 66369-1-Ig

Synonyme

PPARGC1A, 1C1B2, LEM6, Ligand effect modulator 6, PGC 1 alpha



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHausschwein-Skelettmuskelgewebe, Maus-Skelettmuskelgewebe, Ratten-Skelettmuskelgewebe
Erfolgreiche Detektion in IF/ICCHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:5000-1:50000
Immunfluoreszenz (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

66369-1-Ig bindet in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA PGC1a und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

Getestete Reaktivität Hausschwein, human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Affe, hamster, Hausschwein, Huhn, Maus, Ratte, Zebrafisch, Ziege
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen Peptid
Vollständiger Name peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Berechnetes Molekulargewicht 91 kDa
Beobachtetes Molekulargewicht 70 kDa, 100 kDa
GenBank-ZugangsnummerNM_013261
Gene symbol PGC1a
Gene ID (NCBI) 10891
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS with 0.02% sodium azide and 50% glycerol
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.

Protokolle

PRODUKTSPEZIFISCHE PROTOKOLLE
WB protocol for PGC1a antibody 66369-1-IgProtokoll herunterladen
IF protocol for PGC1a antibody 66369-1-IgProtokoll herunterladen
STANDARD-PROTOKOLLE
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
mouseWB

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
mouseWB

Nat Aging

Activation of AMPK by GLP-1R agonists mitigates Alzheimer-related phenotypes in transgenic mice

Authors - Yun Zhang
mouseWB

Nat Commun

Moderate-intensity interval exercise exacerbates cardiac lipotoxicity in high-fat, high-calories diet-fed mice

Authors - Jing Geng
humanChIP

Adv Sci (Weinh)

Sirtuin1 Suppresses Calcium Oxalate Nephropathy via Inhibition of Renal Proximal Tubular Cell Ferroptosis Through PGC-1α-mediated Transcriptional Coactivation

Authors - Chen Duan
mouseWB

Sci Adv

Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice.

Authors - Jun Seok Son
chickenWB,IF

J Hazard Mater

Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers

Authors - Gaolong Zhong

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Michaela (Verified Customer) (11-24-2023)

Worked well in 15μg mouse brain tissue lysate in Western Blot. Band appears between 60-100 kDa

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse brain tissue
FH

No (Verified Customer) (10-24-2023)

A good antibody to detect endogenous PGC1a.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
PGC1a Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:66369-1-Ig)
FH

Mi (Verified Customer) (03-07-2023)

It doesn´t work well in human white and brown adipocyte cells, we got bands at unexpected size. Further KO validation may be needed.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: adipocytes
FH

Huai-Chin (Verified Customer) (07-11-2022)

There are several non-specific bands. At least there's a dominant band after adipogenesis and we can use this antibody at a fairly high dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: 3T3-L1 cells
FH

Mohammad (Verified Customer) (01-21-2020)

It was working with some non-specific bands in lower weight. But after 9 months antibody was degraded in -20.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse hippocampus