PPCDC Polyklonaler Antikörper

PPCDC Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16270-1-AP

Synonyme

CoaC, MDS018, PPC DC, PPCDC, UNQ9365/PRO34154

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  WB analysis of human placenta using 16270-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16270-1-AP (PPCDC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16270-1-AP (PPCDC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HL-60-Zellen, humanes Plazenta-Gewebe, K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

16270-1-AP bindet in WB, ELISA PPCDC und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPCDC fusion protein Ag9337
• Vollständiger Name: phosphopantothenoylcysteine decarboxylase
• Berechnetes Molekulargewicht: 204 aa, 22 kDa
• Beobachtetes Molekulargewicht: 22-25 kDa, 14 kDa
• GenBank-Zugangsnummer: BC014409
• Gene symbol: PPCDC
• Gene ID (NCBI): 60490
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC, one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine. The antibody was generated against full length human recombinant PPCDC, which is a bright yellow protein, indicating the presence of flavins. The antibody solution gives yellow color, too. It may be caused by elution of flavins during affinity purification with PPCDC-protein column.