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PPCDC Polyklonaler Antikörper

PPCDC Polyklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16270-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of human placenta tissue using PPCDC Polyclonal antibody (16270-1-AP)

    WB analysis of human placenta using 16270-1-AP (same clone as 16270-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16270-1-AP (PPCDC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16270-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPCDC Polyclonal antibody (16270-1-AP)

    WB analysis using 16270-1-AP (same clone as 16270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16270-1-AP (PPCDC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16270-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

16270-1-PBS bindet in WB, Indirect ELISA PPCDC und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PPCDC fusion protein Ag9337
Vollständiger Name phosphopantothenoylcysteine decarboxylase
Berechnetes Molekulargewicht 204 aa, 22 kDa
Beobachtetes Molekulargewicht22-25 kDa, 14 kDa
GenBank-ZugangsnummerBC014409
Gene symbol PPCDC
Gene ID (NCBI) 60490
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC, one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine. The antibody was generated against full length human recombinant PPCDC, which is a bright yellow protein, indicating the presence of flavins. The antibody solution gives yellow color, too. It may be caused by elution of flavins during affinity purification with PPCDC-protein column.