PPDPF Polyklonaler Antikörper

PPDPF Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 19912-1-PBS

Synonyme

C20orf149, EXPDF

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  WB analysis using 19912-1-AP (same clone as 19912-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19912-1-AP (PPDPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 19912-1-AP (same clone as 19912-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19912-1-AP (PPDPF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 19912-1-AP (same clone as 19912-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19912-1-AP (C20orf149 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 19912-1-AP (same clone as 19912-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19912-1-AP (PPDPF antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 19912-1-AP (same clone as 19912-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19912-1-AP (PPDPF antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 19912-1-AP (same clone as 19912-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PPDPF antibody (19912-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

19912-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PPDPF und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPDPF fusion protein Ag13740
• Vollständiger Name: chromosome 20 open reading frame 149
• Berechnetes Molekulargewicht: 114 aa, 12 kDa
• Beobachtetes Molekulargewicht: 10-12 kDa
• GenBank-Zugangsnummer: BC002531
• Gene symbol: PPDPF
• Gene ID (NCBI): 79144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pancreatic progenitor cell differentiation and proliferation factor (PPDPF), also known as C20orf149 and EXPDF, belongs to the PPDPF family. PPDPF is a key regulator for the development of exocrine pancreas. PPDPF is highly expressed in various cancers, including HCC, colorectal cancer, prostate cancer, etc (PMID: 34031390, 34975328, 37027301). PPDPF consists of 114 amino acids and the molecular weight is 12 kDa.