PPID Polyklonaler Antikörper

PPID Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(12)

Kat-Nr. : 12716-1-AP

Synonyme

CYP40, Cyclophilin-related protein, Cyclophilin-40, Cyclophilin D, Cyclophilin 40

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  WB analysis of mouse brain using 12716-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12716-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12716-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12716-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12716-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12716-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12716-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12716-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12716-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12716-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12716-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12716-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 12716-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12716-1-AP (PPID antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12716-1-AP

  IP result of anti-PPID (IP:12716-1-AP, 4ug; Detection:12716-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of human cervical cancer using 12716-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12716-1-AP (PPID antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse testis using 12716-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 12716-1-AP (PPID antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 12716-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 12716-1-AP (PPID antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of K-562 using 12716-1-AP

  Immunofluorescent analysis of (4% PFA) fixed K-562 cells using PPID antibody (12716-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, A549-Zellen, humanes Hirngewebe, Jurkat-Zellen, MCF-7-Zellen, NIH/3T3-Zellen, Raji-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe, humanes Zervixkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: K-562-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:250-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 12 publications below
• IF: See 1 publications below
• CoIP: See 3 publications below

Produktinformation

12716-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PPID und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPID fusion protein Ag3409
• Vollständiger Name: peptidylprolyl isomerase D
• Berechnetes Molekulargewicht: 370 aa, 41 kDa
• Beobachtetes Molekulargewicht: 38-41 kDa
• GenBank-Zugangsnummer: BC030707
• Gene symbol: PPID
• Gene ID (NCBI): 5481
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPID(Peptidyl-prolyl cis-trans isomerase D) is also named CYP40, CYPD, and belongs to the cyclophilin-type PPIase family, which accelerates the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. The inactivation of the Ppid gene rescued the disease phenotype of Col6a1 deficiency (PMID:19293339). This PPID antibody detected a strong band at an apparent molecular mass of 41 kDa and a weak band at 70 kDa due to crosslink (PMID: 33320090, 29097228).

Publikationen

Species	Application	Title
	WB	Front Mol Neurosci; PMID: 29632473; Authors - Jian Zhang
rat	WB,CoIP	Cardiovasc Toxicol; PM2.5-Induced Programmed Myocardial Cell Death via mPTP Opening Results in Deteriorated Cardiac Function in HFpEF Mice.; Authors - Tingting Wu
human	WB	Mol Med Rep; Downregulation of microRNA‑199a‑5p attenuates hypoxia/reoxygenation‑induced cytotoxicity in cardiomyocytes by targeting the HIF‑1α‑GSK3β‑mPTP axis.; Authors - Da-Wei Liu
human	WB	Eur J Pharmacol; A novel ent-kaurane diterpenoid analog, DN3, selectively kills human gastric cancer cells via acting directly on mitochondria.; Authors - Yong-Cheng Ma
human	WB	Molecules; The Mechanism of a Novel Mitochondrial-Targeted Icaritin Derivative in Regulating Apoptosis of BEL-7402 Cells Based on the SIRT3 and CypD-Mediated ROS/p38 MAPK Signaling Pathway; Authors - Zenan Chen
human	WB,CoIP	J Periodontal Res; Porphyromonas gingivalis Induces Endothelial Dysfunction Through Sirt3-Dependent CypD Acetylation; Authors - Shengming Xu