PPIF Polyklonaler Antikörper

PPIF Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18466-1-PBS

Synonyme

PPIF-Specific, Cyclophilin D, Cyclophilin F, Cyp D, CyP-D

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  WB analysis using 18466-1-AP (same clone as 18466-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 18466-1-AP (same clone as 18466-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

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  WB analysis of human heart using 18466-1-AP (same clone as 18466-1-PBS)

  human heart tissue were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

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  WB analysis of rat heart using 18466-1-AP (same clone as 18466-1-PBS)

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18466-1-AP (PPIF-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse heart using 18466-1-AP (same clone as 18466-1-PBS)

  IP result of anti-PPIF (IP:18466-1-AP, 3ug; Detection:18466-1-AP 1:1000) with mouse heart tissue lysate 4000ug. This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (PPIF Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 18466-1-AP (CYPA Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 18466-1-AP (PPIF-Specific antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 18466-1-AP (same clone as 18466-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart using 18466-1-AP (PPIF-Specific antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 18466-1-AP (same clone as 18466-1-PBS)

  Immunofluorescent analysis of MCF-7 cells, using PPIF antibody 18466-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 18466-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18466-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PPIF und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPIF fusion protein Ag13151
• Vollständiger Name: peptidylprolyl isomerase F
• Berechnetes Molekulargewicht: 22 kDa
• Beobachtetes Molekulargewicht: 22 kDa
• GenBank-Zugangsnummer: BC005020
• Gene symbol: PPIF
• Gene ID (NCBI): 10105
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPIF, also named as Cyclophilin D (CypD), CYP3, PPIase F and Rotamase F, belongs to the cyclophilin-type PPIase family. PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. PPIF is a mitochondrial matrix protein involved in the induction of necrotic and apoptotic cell death through the activation of the mitochondrial permeability transition (mPT) pore. This antibody is immunized with full length gene PPIF and absorbed by PPIA. So this antibody is specific to PPIF.