PPIL1 Polyklonaler Antikörper

PPIL1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15144-1-PBS

Synonyme

CGI 124, CGI-124, CYPL1, EC:5.2.1.8, hCyPX

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  WB analysis of NIH/3T3 using 15144-1-AP (same clone as 15144-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15144-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 15144-1-AP (same clone as 15144-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15144-1-PBS in a different storage buffer formulation.

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  WB analysis of A375 using 15144-1-AP (same clone as 15144-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 15144-1-AP (PPIL1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15144-1-PBS in a different storage buffer formulation.

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  IP experiment of NIH/3T3 using 15144-1-AP (same clone as 15144-1-PBS)

  IP result of anti-PPIL1 (IP:15144-1-AP, 3ug; Detection:15144-1-AP 1:400) with NIH/3T3 cells lysate 1600ug. This data was developed using the same antibody clone with 15144-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15144-1-AP (same clone as 15144-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15144-1-AP (PPIL1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15144-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 15144-1-AP (same clone as 15144-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 15144-1-AP (PPIL1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15144-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 15144-1-AP (same clone as 15144-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 15144-1-AP (PPIL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15144-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15144-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA PPIL1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPIL1 fusion protein Ag7436
• Vollständiger Name: peptidylprolyl isomerase (cyclophilin)-like 1
• Berechnetes Molekulargewicht: 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC003048
• Gene symbol: PPIL1
• Gene ID (NCBI): 51645
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Peptidyl-prolyl isomerase (PPIase) catalyzes the interconversion of a specific Pro-imide bond between the cis and trans conformations. PPIL1 (Peptidyl-prolyl cis-trans isomerase-like 1) is up-regulated in human colon cancer cells and an siRNA-mediated knockdown of PPIL1 resulted in a reduced number of viable cell (PMID: 20368803). Expression of PPIL1 was shown to be ubiquitous across all adult tissues (with highest expression in the adrenal gland, testis and heart). PPIL1 is also known to participate in the spliceosome; a complex and dynamic protein and RNA complex responsible for splicing introns from pre-mRNA (PMID: 33220177, 8978786).