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PPIP5K1 Polyklonaler Antikörper

PPIP5K1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14331-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using PPIP5K1 Polyclonal antibody (14331-1-AP)

    WB analysis using 14331-1-AP (same clone as 14331-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14331-1-AP (PPIP5K1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14331-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPIP5K1 Polyclonal antibody (14331-1-AP)

    IHC staining of mouse skeletal muscle using 14331-1-AP (same clone as 14331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14331-1-AP (PPIP5K1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14331-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPIP5K1 Polyclonal antibody (14331-1-AP)

    IHC staining of mouse skeletal muscle using 14331-1-AP (same clone as 14331-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 14331-1-AP (PPIP5K1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14331-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using PPIP5K1 Polyclonal antibody (14331-1-AP)

    IF Staining of U-251 using 14331-1-AP (same clone as 14331-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using PPIP5K1 antibody (14331-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14331-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

14331-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PPIP5K1 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen PPIP5K1 fusion protein Ag5614
Vollständiger Name histidine acid phosphatase domain containing 2A
Berechnetes Molekulargewicht 160 kDa
Beobachtetes Molekulargewicht160 kDa
GenBank-ZugangsnummerBC050263
Gene symbol HISPPD2A
Gene ID (NCBI) 9677
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPIP5K1 (Inositol diphosphoinositol pentakisphosphate kinase type 1) plays an important role in regulating cell death in response to genotoxic stress. PPIP5K1 act in concert with IP6K2 to promote cell death through controlling localization of stress-activated MAP kinase-interacting protein1 (SIN1) (PMID: 31051014). The activation of the PtdIns 3-kinase pathway is associated with PPIP5K1 translocation from the cytoplasm to the plasma membrane (PMID: 21222653).