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PPM1B Monoklonaler Antikörper

PPM1B Monoklonal Antikörper für WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

2C7H2

Kat-Nr. : 67647-1-PBS

Synonyme

2C7H2, EC:3.1.3.16, PP2C beta, PP2CB, PP2C-beta

Filter:
  • Western Blot (WB) analysis of various lysates using PPM1B Monoclonal antibody (67647-1-Ig)

    WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPM1B Monoclonal antibody (67647-1-Ig)

    WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Sample test of MP51322-1

    Sample test of MP51322-1

    Sample test of MP51322-1, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-2-PBS.

  • Sample test of MP51322-2

    Sample test of MP51322-2

    Sample test of MP51322-2, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-3-PBS.

  • Sample test of MP51322-3

    Sample test of MP51322-3

    Sample test of MP51322-3, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-4-PBS.

  • Sample test of MP51322-4

    Sample test of MP51322-4

    Sample test of MP51322-4, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-5-PBS.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of mouse skeletal muscle using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using PPM1B Monoclonal antibody (67647-1-Ig)

    IHC staining of mouse skeletal muscle using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using PPM1B Monoclonal antibody (67647-1-Ig)

    IF Staining of A431 using 67647-1-Ig (same clone as 67647-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PPM1B antibody (67647-1-Ig, Clone: 2C7H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP51322-4

    Cytometric bead array standard curve of MP51322-4

    Cytometric bead array standard curve of MP51322-4, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-5-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

  • Cytometric bead array standard curve of MP51322-1

    Cytometric bead array standard curve of MP51322-1

    Cytometric bead array standard curve of MP51322-1, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-2-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

  • Cytometric bead array standard curve of MP51322-2

    Cytometric bead array standard curve of MP51322-2

    Cytometric bead array standard curve of MP51322-2, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-3-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

  • Cytometric bead array standard curve of MP51322-3

    Cytometric bead array standard curve of MP51322-3

    Cytometric bead array standard curve of MP51322-3, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-4-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

  • Affinity and Kinetic Characterization of 67647-1-Ig

    Affinity and Kinetic Characterization of 67647-1-Ig

    Biolayer interferometry (BLl) kinetic assay of 67647-1-Ig against Human PPM1B was performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

67647-1-PBS bindet in WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test PPM1B und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen PPM1B fusion protein Ag3871
Vollständiger Name protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
Berechnetes Molekulargewicht 479 aa, 53 kDa
Beobachtetes Molekulargewicht 36, 53 kDa
GenBank-ZugangsnummerBC012002
Gene symbol PPM1B
Gene ID (NCBI) 5495
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.