PPM1G Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PPM1G Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Kat-Nr. : 15532-1-AP

Synonyme

PP2C gamma, PP2CG, PP2CGAMMA, PPM1C, PPM1G, PPP2CG, Protein phosphatase 1C, Protein phosphatase 1G

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Galerie der Validierungsdaten

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  WB analysis using 15532-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15532-1-AP (PPM1G antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 15532-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15532-1-AP (PPM1G antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 15532-1-AP (PPM1G antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 15532-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15532-1-AP (PPM1G antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 15532-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (15532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 15532-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPM1G antibody (15532-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293T-Zellen, Jurkat-Zellen, Mauslungengewebe, MCF-7-Zellen
• Erfolgreiche Detektion in IHC: Mausnierengewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Lebergewebe, humanes Nierengewebe, humanes Plazenta-Gewebe, Maushodengewebe, Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Produktinformation

15532-1-AP bindet in WB, IHC, IF, ELISA PPM1G und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPM1G fusion protein Ag7751
• Vollständiger Name: protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC000057
• Gene symbol: PPM1G
• Gene ID (NCBI): 5496
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPM1G, also known as PP2Cγ and PP2CG, is an important member of the PP2C family of serine/threonine protein phosphatases and has an important role in controlling cell cycle progression. PPM1G restricts innate immune signaling mediated by STING and MAVS and is hijacked by KSHV for immune evasio(PMID: 33219031). In addition, PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3(PMID: 34290239).

Publikationen

Species	Application	Title
human	WB	Br J Pharmacol; Costunolide represses hepatic fibrosis through WWP2-mediated Notch3 degradation.; Authors - Mao-Xu Ge
human	WB	J Oncol; Identifying Potential Tumor Antigens and Antigens-Related Subtypes in Hepatocellular Carcinoma for mRNA Vaccine Development; Authors - Weiran Liao
human	WB,IHC	Cell Death Dis; PPM1G promotes the progression of hepatocellular carcinoma via phosphorylation regulation of alternative splicing protein SRSF3.; Authors - Dawei Chen ; KD Validated