PDP1 Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

PDP1 Rekombinant Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242090E7

Kat-Nr. : 84612-3-PBS

Synonyme

PPM2C, [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial, 242090E7, EC:3.1.3.43, PDP 1

---

• 

  WB analysis of PC-3 using 84612-3-RR (same clone as 84612-3-PBS)

  WB result of PDP1 antibody (84612-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDP1 transfected PC-3 cells. This data was developed using the same antibody clone with 84612-3-PBS in a different storage buffer formulation.

• 

  WB analysis using 84612-3-RR (same clone as 84612-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84612-3-RR (PDP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84612-3-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 84612-3-RR (same clone as 84612-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84612-3-RR (PDP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84612-3-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 84612-3-RR (same clone as 84612-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84612-3-RR (PDP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84612-3-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01450-2

  Cytometric bead array standard curve of MP01450-2, PPM2C Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84612-1-PBS. Detection antibody: 84612-3-PBS. Standard: Ag15453. Range: 2.5-80 ng/mL.

• 

  Affinity and Kinetic Characterization of 84612-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 84612-3-PBS against Human PDP1 was performed. The affinity constant is 358 nM.

• 

  Affinity and Kinetic Characterization of 84612-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84612-3-RR against Human PDP1 were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

84612-3-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA PDP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDP1 fusion protein Ag15453
• Vollständiger Name: protein phosphatase 2C, magnesium-dependent, catalytic subunit
• Berechnetes Molekulargewicht: 537 aa, 61 kDa
• Beobachtetes Molekulargewicht: 55-61 kDa
• GenBank-Zugangsnummer: BC047619
• Gene symbol: PDP1
• Gene ID (NCBI): 54704
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pyruvate dehydrogenase phosphatase 1 (PDP1), also known as PPM2C, is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).