PDP1 Rekombinanter Antikörper

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• KD/KO Validated

PDP1 Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

242090E7

Kat-Nr. : 84612-3-RR

Synonyme

PPM2C, [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial, 242090E7, EC:3.1.3.43, PDP 1

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  WB analysis of PC-3 using 84612-3-RR

  WB result of PDP1 antibody (84612-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDP1 transfected PC-3 cells.

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  WB analysis using 84612-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84612-3-RR (PDP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 84612-3-RR

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84612-3-RR (PDP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 84612-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84612-3-RR (PDP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 84612-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84612-3-RR against Human PDP1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, Mausherzgewebe, Maus-Skelettmuskelgewebe, PC-3-Zellen, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84612-3-RR bindet in WB, IHC, ELISA PDP1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDP1 fusion protein Ag15453
• Vollständiger Name: protein phosphatase 2C, magnesium-dependent, catalytic subunit
• Berechnetes Molekulargewicht: 537 aa, 61 kDa
• Beobachtetes Molekulargewicht: 55-61 kDa
• GenBank-Zugangsnummer: BC047619
• Gene symbol: PDP1
• Gene ID (NCBI): 54704
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pyruvate dehydrogenase phosphatase 1 (PDP1), also known as PPM2C, is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).