PPME1 Polyklonaler Antikörper

PPME1 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 14435-1-AP

Synonyme

EC:3.1.1.89, PME 1, PME1, PME-1, PP2593

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  WB analysis using 14435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14435-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14435-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14435-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 14435-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14435-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 14435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A549-Zellen, fetales humanes Hirngewebe, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:4000-1:20000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

14435-1-AP bindet in WB, IHC, ELISA PPME1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPME1 fusion protein Ag5817
• Vollständiger Name: protein phosphatase methylesterase 1
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC050705
• Gene symbol: PPME1
• Gene ID (NCBI): 51400
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPME1 (Protein phosphatase methylesterase 1) is a protein phosphatase 2A (PP2A)-specific methyl esterase that negatively regulates PP2A through demethylation at its carboxy terminal leucine 309 residue (PMID: 24253382). PPME1 plays an important role in the development of cancers and may be a biomarker for pancreatic carcinoma, gastric and lung cancer (PMID: 32351291).

Publikationen

Species	Application	Title
human	WB	Front Immunol; CBP Bromodomain Inhibition Rescues Mice From Lethal Sepsis Through Blocking HMGB1-Mediated Inflammatory Responses.; Authors - Xiaowen Bi
human	WB	Neural Regen Res; FUBP3 mediates the amyloid-β-induced neuronal NLRP3 expression; Authors - Jing Yao