PPME1 Polyklonaler Antikörper

PPME1 Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14435-1-PBS

Synonyme

EC:3.1.1.89, PME 1, PME1, PME-1, PP2593

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  WB analysis using 14435-1-AP (same clone as 14435-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 14435-1-AP (same clone as 14435-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 14435-1-AP (same clone as 14435-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14435-1-AP (same clone as 14435-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 14435-1-AP (same clone as 14435-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14435-1-AP (same clone as 14435-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14435-1-AP (same clone as 14435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 14435-1-AP (same clone as 14435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 14435-1-AP (same clone as 14435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14435-1-PBS bindet in WB, IHC, Indirect ELISA PPME1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PPME1 fusion protein Ag5817
• Vollständiger Name: protein phosphatase methylesterase 1
• Berechnetes Molekulargewicht: 42 kDa
• Beobachtetes Molekulargewicht: 44 kDa
• GenBank-Zugangsnummer: BC050705
• Gene symbol: PPME1
• Gene ID (NCBI): 51400
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPME1 (Protein phosphatase methylesterase 1) is a protein phosphatase 2A (PP2A)-specific methyl esterase that negatively regulates PP2A through demethylation at its carboxy terminal leucine 309 residue (PMID: 24253382). PPME1 plays an important role in the development of cancers and may be a biomarker for pancreatic carcinoma, gastric and lung cancer (PMID: 32351291).