PPP1CB-Specific Polyklonaler Antikörper

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• KD/KO Validated

PPP1CB-Specific Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Rezensionen (1)

Kat-Nr. : 55136-1-AP

Synonyme

PPP1CB, PPP1CD, PP-1B, PP1 Beta, PP 1B

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  WB analysis using 55136-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skin cancer using 55136-1-AP

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 55136-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A549 cells using 55136-1-AP (PPP1CB-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A549-Zellen, BxPC-3-Zellen, Maushirngewebe, NIH/3T3-Zellen, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IHC: humanes Hautkrebsgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: A549-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunhistochemie (IHC): IHC : 1:400-1:1600
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below

Produktinformation

55136-1-AP bindet in WB, IHC, IF/ICC, ELISA PPP1CB-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: protein phosphatase 1, catalytic subunit, beta isoform
• Berechnetes Molekulargewicht: 37 kDa
• Beobachtetes Molekulargewicht: 33-40 kDa
• GenBank-Zugangsnummer: NM_002709
• Gene symbol: PPP1CB
• Gene ID (NCBI): 5500
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PPP1CB, also named as PP-1B, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase (PP1) is essential for cell division, it participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. PPP1CB is involved in regulation of ionic conductances and long-term synaptic plasticity. This antibody is specific to PPP1CB.

Publikationen

Species	Application	Title
mouse	WB	J Adv Res; Transducin-like enhancer of split 3 protects against lipopolysaccharide-induced inflammation through DDX5-ATF1-PPP2R5A signaling; Authors - Di Fan
human	WB	PLoS Pathog; Protein phosphatase 1 catalyzes HBV core protein dephosphorylation and is co-packaged with viral pregenomic RNA into nucleocapsids.; Authors - Zhanying Hu ; KD Validated
human	WB	Exp Eye Res; PPP1CA/YAP/GS/Gln/mTORC1 pathway activates retinal Müller cells during diabetic retinopathy.; Authors - Yang Guo

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Balawant (Verified Customer) (08-23-2022)

I have used this antibody for western blot in mice tissue sample (colon tissue). it is working great.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Colon tissue sample and caco2 cells